16 Jun Chapter 8 – Prokaryotic genome structure
Question
Chapter 8
Prokaryotic genome structure (chromosome features, origin of replication, operons, genes)
DNA replication (semiconservative, and relationship to genome structure)
Operon model of transcriptional regulation (inducible, repressible and catabolite repression)
Be able to transcribe a gene and translate a mRNA
Mutations (types and effects: on protein products and on protein function)
Nucleoside analogs
Horizontal Gene Transfer: Conjugation, transduction and transformation
Role of recombination in horizontal gene transfer
Chapter 9
Cloning a gene
PCR: components required, process, thermal cycling
Restriction enzymes, how they work, how they are used, their normal cellular purpose
Cloning vectors (selection, restriction sites, origin of replication)
Chapter 13
HPV
Oncogenic viruses, oncogenes and tumor suppressor genes
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