13 Jul DO NOT DO RESULT SECTION This lab report will cover the transforma
DO NOT DO RESULT SECTION This lab report will cover the transformation of E.coli and the restriction digest of the plasmid (labs 5 to 8).Use the Standard lab report format and include the following – Overall objective – Background to what you were trying to do – Methods – used to create reagents and used for the transformation, plasmid isolation, restriction digest and agarose gels. Create sub section for each Transformation- What was the selectable marker?- How do you know you successfully transformed the cells with pGlo- What was the most critical step in the transformation processPlasmid preparation- Why does only the E. coli grown in ara/amp fluoresceRestriction digest – What size fragments would you expect when cutting the plasmid with the two enzymes used? – Are the results of the plasmid what you expected.- Are the two fragments the sizes you expected ?
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