12 Jun Edinboro BIOL 300 – EXPLORING GENETICS
Question
EXPLORING GENETICS
In these exercise you will explore how organisms pass on heritable traits from one
generation to the next. In doing so, you will explore the basic principles of genetics, the
science of inheritance. Since the dawn of history, people have been curious about how
organisms pass on certain traits from generation to generation. You will begin to
investigate the inheritance of a specific trait and its variation. Couple #1 has straight ears.
Couple #2 has floppy ears.
Question #1: What is the trait and what are its variations?
When a rabbit with straight ears is crossed with another rabbit with straight ears, one will
always see straight ears in the P, F1, F2, F3, and subsequent generations.
Question #2: How many trait variations do you see in the F1, F2, and subsequent
generations of offspring? Please explain your subsequent answers using the following
terms: (hint: use terms in questions 21-23 to assist in your answers)
Trait – a distinguishing quality or characteristic
Genetic Variation – Genetic variation
can refer to
differences between individuals or to
differences between populations.
Question #3: How does this compare with the trait variations of the parents, Couple #1?
Question #4: What is the number of trait variations in the F2 offspring?
Question #5: How does this compare with the trait variation of their parents (the F1
offspring)?
When a rabbit with floppy ears is crossed with another rabbit with floppy ears, one will
always see floppy ears in the P, F1, F2, F3, and subsequent generations.
Question #6: How many trait variations do you see in the F1 offspring?
Question #7: How does this compare with the trait variations of the parents, Couple #2?
Question #8: What is the number of trait variations in the F2 offspring?
Question #9: How does this compare with the trait variation of their parents (the F1
offspring)?
Question #10: How might you characterize the line of generations, beginning with
couple #1, with respect to the trait that you are testing?
Question #11: How might you characterize the line descending from couple #2?
Question #12: How might you characterize the individuals in each line?
Mate a male couple #1 to a female couple #2. You see all straight ears in the F1.
Question #13: Describe the observed ear position you see in the offspring and compare
with the parents.
Question #14: What do you find puzzling about the F1 offspring as compared with their
parents?
Mate two (2) F1 straight eared individuals. With a sample size of 1000, what results do
you expect in the F2 generation?
Question #15: How many types of ears do you expect to see in the F2 generation? Why?
Question #16: What is puzzling/not puzzling about these results?
Question #17: Do your expected results apply generally in the F2 generation?
Question #18: Describe the expected results in terms of the parents, F1, and F2
generations.
Question #19: Can you suggest an explanation for these results?
Question #20: How do these results compare with Mendel’s strategy?
Question #21: Did a trait variation found in one of the parents disappear in the F1
offspring?
Question #22: If so, what was this trait variation?
Question #23: Was this trait variation actually "lost"?
Question #24: How might you explain the observation that this variation disappeared in
the F1 offspring?
Question #25: In the F1 generation in your simulation, which trait variation could be
called dominant?
Question #26: Which trait variation could be called recessive?
Question #27: How many alleles are carried by an individual for each trait?
Question #28: What were the genotypes (allelic constitution – the particular alleles,
or alternate forms of a gene, at specified loci present in an organism) and
phenotypes of the individuals in the P generation?
Question #29: What were the genotypes of all the F1 offspring?
Now you will follow and test Mendel’s reasoning. First, you will take a closer look at the
ratio of the phenotypes in the F2 offspring, beginning with purebred individuals. The trait
to be studied is ear position. Crossing a purebred straight eared rabbit with a purebred
floppy eared rabbit (with a litter size of 100), you receive the following results in the F2
generations: 75 straight ears, 25 floppy ears.
Question #1: Which trait variation is dominant?
Question #2: Which trait variation is recessive?
Question #3: What is the ratio of F2 offspring? (note: ratios are expressed as a
number:1). You can round off to the nearest whole number.
Question #4: Calculate the expected genotypic ratios.
Question #5: Can you distinguish Aa from AA by looking at their phenotypes?
Question #6: Design a cross (crosses) that would distinguish between AA and Aa.
10 F2 individuals having the dominant phenotype were crossed with an individual of
known genotype. Results of these crosses were obtained:
INDIVIDUAL
STRAIGHT
FLOPPY
1
100
0
2
54
46
3
58
42
4
100
0
5
48
52
6
51
49
7
100
0
8
45
55
9
53
47
10
50
50
Question #7: How many individuals of genotype AA and Aa did you identify among the
10 F2 individuals examined?
Question #8: If Mendel’s hypothesis was correct, based on your F1 Punnett square, what
ratio of AA to Aa individuals would you expect in the F2 generation?
Question #9: Do these results confirm Mendel’s hypothesis?
Question #10: How does a test cross verify Mendel’s hypothesis?
Question #11: A normal couple who are both heterozygous for the gene for cystic
fibrosis (this gene is located on an autosome) have two children who have cystic fibrosis.
What is the probability that their next child will have cystic fibrosis? Please show all
work (Punnett Square) to support your answer.
Question #12: If the allele for inflated pea pods (I) is dominant to the allele for constricted (i),
the cross Ii X ii is expected to produce:
a.
All with inflated pods b. All with constricted pods
c.
Half with inflated and half with constricted pods
d.
3/4 with inflated and 1/4 with constricted pods
e.
3/4 with constricted and 1/4 with inflated pods
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