25 Jun Experiment 1: Effects of pH on Radish Seed Germination Natural
Question
Experiment 1: Effects of pH on Radish Seed Germination
Natural soil pH depends on the parent rock material from which it was formed and processes like climate. Soil pH is a measure of the acidity or alkalinity of the soil. Acidic soils are considered to have a 5.0 or lower pH value whereas 10.0 or above is considered a strong basic or alkaline soil. The pH of soil affects the solubility of nutrients in soil water and thus it affects the amount of nutrients available for plant uptake. Different nutrients are available under differing pH conditions.
.jpg”>In this experiment we will look at the effect of pH on the germination and growth rate of radish seeds in order to determine the range of pH tolerance for the seed. Acidic or basic water will be used in order to stimulate acidity or alkalinity in soil.
Materials
2 mL 4.5% Acetic Acid (Vinegar), C2H4O2
Permanent Marker
(3) 5 cm Petri Dishes
3 pH Test Strips
Radish Seed Packet
Ruler
2 mL 15% Saturated Sodium Bicarbonate
(Baking Soda) Solution, NaHCO3
*Paper Towel Sheets (cut to fit into the petri dish)
*Scissors
*Sunny Location
*Water
*You Must Provide
Procedure
Use the permanent marker to label the top of each of the three petri dishes as Acetic Acid, Sodium Bicarbonate, or Water.
Carefully cut three small circles from the paper towel sheets. The circles should comfortably fit within the bottom of the petri dish.
Place the circles in the dishes, and wet them with approximately 2 mL of each respective solution (acetic acid, sodium bicarbonate, or water).
Gently press the reaction pad of three, pH test strips onto the wet paper towels. Record your data in Table 1.
Arrange 10 radish seeds on each paper towel in each petri dish. Make sure the seeds have space and are not touching. Then, place the top of the petri dish on the bottom.
Create a hypothesis regarding which environment will provide the most hearty radish seed growth. Be sure to indicate why you believe this will be true, and use scientific reasoning. Record your hypothesis in Post-Lab Question 1.
Place the petri dishes in a sunny or well-lit, warm place. Be sure to keep the paper towels moist for the length of the experiment with the appropriate solution if any of the towels dry out.
Observe the seeds daily for seven days, and record the number of seeds that germinate in Table 1. Note when the seeds crack and roots or shoots emerge). On the seventh day, record the lengths of radish seed sprouts (mm or cm).
Table 1: pH and Radish Seed Germination
Stage/Day Observations
Acetic Acid
Sodium Bicarbonate
Water
Initial pH
1
2
3
4
5
6
7
Post-Lab Questions
Record your hypothesis from Step 6 here:
Construct a line graph based on the data from Table 1 in the space below. Place the day on the x axis, and the number of seeds germinated on the y axis. Be sure to include a title, label the x and y axes, and provide a legend describing which line corresponds to each plate (e.g., blue = acetic acid, green = sodium bicarbonate, etc…).
Was there any noticeable effect on the germination rate of the radish seeds as a result of the pH? Compare and contrast the growth rate for the control with the alkaline and acidic solutions.
According to your results would you say that the radish has a broad pH tolerance? Why or why not? Use your data to support your answer.
Knowing that acid rain has a pH of 2-3 would you conclude that crop species with a narrow soil pH range are in trouble? Is acid rain a problem for plant species and crops?
Research and briefly describe a real world example about how acid rain affect plants. Be sure to demonstrate how pH contributes to the outcome, and proposed solutions (if any). Descriptions should be approximately 2 – 3 paragraphs. Include at least three citations (use APA formatting).
Our website has a team of professional writers who can help you write any of your homework. They will write your papers from scratch. We also have a team of editors just to make sure all papers are of HIGH QUALITY & PLAGIARISM FREE. To make an Order you only need to click Ask A Question and we will direct you to our Order Page at WriteDemy. Then fill Our Order Form with all your assignment instructions. Select your deadline and pay for your paper. You will get it few hours before your set deadline.
Fill in all the assignment paper details that are required in the order form with the standard information being the page count, deadline, academic level and type of paper. It is advisable to have this information at hand so that you can quickly fill in the necessary information needed in the form for the essay writer to be immediately assigned to your writing project. Make payment for the custom essay order to enable us to assign a suitable writer to your order. Payments are made through Paypal on a secured billing page. Finally, sit back and relax.
About Writedemy
We are a professional paper writing website. If you have searched a question and bumped into our website just know you are in the right place to get help in your coursework. We offer HIGH QUALITY & PLAGIARISM FREE Papers.
How It Works
To make an Order you only need to click on “Order Now” and we will direct you to our Order Page. Fill Our Order Form with all your assignment instructions. Select your deadline and pay for your paper. You will get it few hours before your set deadline.
Are there Discounts?
All new clients are eligible for 20% off in their first Order. Our payment method is safe and secure.