23 Jul EXPLAIN YOUR REASONING BOX 1 > BOX 2> BOX 3 > NR1D1 BOXES 1 2 3 ARE EMPTY.
The expression of the transcription factor HNF6 results in an increase of the expression of the NR1D Show more The expression of the transcription factor HNF6 results in an increase of the expression of the NR1D1 gene. Three sets of fluorescent DNA probes purified by are applied to the DNA arrays. The hybridization of these fluorescent probes with spotted genomic DNA corresponding to the promoter regions of the genes HNF1 HNF4 and NR1D1 are showed in figure 4. Fluorescent probe purified with anti-HNF6 HNF1 HNF4 NR1D1 clear circle black circle clear circle Fluorescent probe purified with anti-HNF4 HNF1 HNF4 NR1D1 black circle clear circle clear circle Fluorescent probe purified with anti-HNF1 HNF1 HNF4 NR1D1 clear circle clear circle Black circle Figure 4: Results of 3 hybridizations with fluorescent DNA fragments purified by immunoprecipitation with anti-HNF6 (left) anti-HNF4 (middle) and anti-HNF1 (right). The names genes whose promoter regions are spotted on the array are indicated on top of each spot. Black circles represent fluorescent spots. The following diagram (see next page) represents a regulatory chain leading to the transcriptional activation of NR1D1. Each box correspond to one of the gene described above and an arrow indicate that the gene on the left side of the arrow activate the transcription of the gene on the left side of the arrow. On the diagram label each box with the name of the corresponding gene. Explain your reasoning Box 1 > box 2> box 3 > NR1D1 Boxes 1 2 3 are empty. Show less
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