11 Jun group porject 3, Mouse cloning
Question
The goal of this group project is to develop a mouse model system for studying how mutations in the murine (mouse) IGF-1 gene might impact body size and lifespan in the mouse. (To learn about IGF-1 and how it functions to regulate growth, longevity, and cancer susceptibility, see the review I have posted in the conference titled “Group Project Reading Material.” Also in this conference, you will find other resources related to molecular and cellular biology techniques.)
As part of this project, your goals are to:
Clone the mouse IGF-1 gene.
Characterize tissue expression patterns of mouse IGF-1 mRNA and protein.
Introduce mutations into the cloned IGF-1 gene, and determine whether these mutations affect expression of the gene*.
Generate an IGF-1 “knockin” mouse. Here, you will “knockin” your mutated IGF-1 gene into the mouse IGF-1 locus so that you can finally determine whether the mutations you have made in the gene will affect mouse size and lifespan.
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