15 Jun how a genetic cross can be used to identify the number of genes
Question
a) Describe, in detail, how a genetic cross can be used to identify the number of genes involved in major changes in body form between two different organisms.
b) If you found a group of similar species living on nearby islands, describe in detail a method you could use to determine their exact evolutionary relationship to each other.
c) Describe how a mutation in the intracellular domain of MC1R could change the coat color in rock pocket from light to dark.
d) Besides looking for microfossils, describe another test you could perform on rocks from 3-4 billion years ago to find evidence that life existed at that time?
Q2.
For the following questions, restriction enzyme digests were performed on a plasmid and the following size fragments were obtained:
Sal 1: 0.2, 1.2 kb & 5.1 kb
Tuf 1: 3.1 kb & 3.4 kb
Sal 1, Tuf 1 double digest: 0.2, 0.3, 0.9, 2.2 and 2.9 kb
a. How many total kb is the intact plasmid?
b.How many times does Sal 1 cut the plasmid?
c. Draw a map of the plasmid with all of the Sal 1 and Tuf 1 recognition sites clearly labeled. Indicate the relative position of the sites by d. labeling the distance between each site in kb.
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