25 Jul HOW MANY COPIES OF A 120 KD PROTEIN WOULD NEED TO BE PRESENT IN A CELL IN ORDER TO BE DETECTABLE?
In this question I am asking you to think about the sensitivity of gel electrophoresis and staining Show more In this question I am asking you to think about the sensitivity of gel electrophoresis and staining. How many copies of a protein need to be present in a typical eukaryotic cell in order for it to be visible as a band on a gel? Assume that you can load 100 ug of protein in a lane and that the faintest band you can detect is 10 ng (by silver staining). The concentration of protein in a cell is about 200 mg/mL and a typical eukaryotic cell has a volume of about 1000 um 3 (1 picoliter). How many copies of a 120 kD protein would need to be present in a cell in order to be detectable? Show less
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