25 Jul HOW MUCH AGAROSE WILL YOU REQUIRE?
100 ml Show more The recipe for one liter of 50X TAE is as follows: 242g Tris base 57.1 ml glacial acetic acid 100 ml 0.5 M EDTA pH 8.0 9. Provide a recipe for 40 ml of 50X TAE. 10. When you make your agarose gel you will need to make 50 ml of 1% (w/v) agarose in 1X TAE. How much agarose will you require? When adding your DNA samples to the agarose gel you will need to add loading dye to the samples. Loading dye contains: glycerol which helps the samples sink into the sample wells of the gel; and tracking dyes so that you have a way of estimating how far the samples have travelled through the gel during electrophoresis. The recipe for 6X loading dye is as follows: 0.25% bromophenol blue (w/v) 0.25% xylene cyanol (w/v) 30% glycerol in water (v/v) 11. You will be asked to make 10 ml of 10X loading dye. Using the information above provide a recipe. (Important tip: Remember that you have already made a 50% stock solution of glycerol. I recommend determining the concentration of glycerol required for 10X loading dye then figuring out how much of your 50% glycerol stock will be needed to make 10ml of 10X loading dye.) Show less
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