23 Jul HOW MUST THESE TWO POPULATIONS HAVE DIFFERED FROM EACH OTHER?
DNA fragments made on the lagging strand were named Okazaki fragment after the researcher who disc Show more DNA fragments made on the lagging strand were named Okazaki fragment after the researcher who discovered these fragments. Okazaki and colleagues discovered the fragments by exposing replicating DNA to radioactively-labeled nucleotides and then separating the labeled DNA fragments by size (experiment description can be found on pg. 369). He found two populations of fragments using this technique. How must these two populations have differed from each other? Why was it important to use a short rather than long exposure to the radioactive label?Magnesium chloride (MgCl2) is added to the buffer used for PCR reactions. Explain why MgCl2 is required for the reaction to occur. Show less
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