In DNA extraction what makes DNA insoluble

10 Jun In DNA extraction what makes DNA insoluble

Question
1) In DNA extraction what makes DNA insoluble and therefore visible?

Select one:

a. isopropyl alcohol

b. proteinase-K

c. salt

d. lysis solution containing detergent

2)
What is the function of primers in PCR?

Select one:

a. raw material for new DNA

b. attach new nucleotides to DNA strand

c. attach to either side of target DNA

d. denature, or separate, DNA

3)
What is the function of DNA polymerase in PCR?

Select one:

a. raw material for new DNA

b. attaches to either side of target DNA

c. attaches new nucleotides to DNA strand

d. denatures, or separates, DNA

4)
What is special about the DNA polymerase used in PCR?

Select one:

a. can withstand low temperatures

b. denatures, or separates, DNA

c. attaches to either side of target DNA

d. can withstand high temperatures

5)
Name the apparatus that will heat and cool the PCR sample.

Select one:

a. PCR tubes

b. micro-centrifuge

c. DNA thermocycler

d. buccal swab

6)
Why is the sample heated to almost the boiling point in PCR?

Select one:

a. the DNA strands pair up

b. primers attach to DNA

c. the DNA denatures, or separates

d. new nucleotides attach to DNA strand

7)
Why is the sample cooled down again in PCR?

Select one:

a. primers attach to DNA

b. to activate DNA polymerase

c. the DNA denatures, or separates

d. the DNA strands pair up

8)
Why is the sample then brought up to 72 degrees Celcius in PCR?

Select one:

a. the DNA strands pair up

b. the DNA denatures, or separates

c. primers attach to DNA

d. to activate DNA polymerase

on

9)
Repeated sequences of DNA that vary in length among different individualsare:

Select one:

a. alleles

b. fluorescent primers

c. restriction enzymes

d. polymorphisms

10)
An STR is longer than a VNTR.

Select one:

True

False

11)
It is extremely unlikely that any two people will have the same profile of repeat polymorphisms.

Select one:

True

False

12)
Each person has two copies of each variable repeat. If they are identical, the person is heterozygous.

Select one:

True

False

13)
What do researchers use to cut specific sites on either side of each repeat region, resulting in fragments of different lengths?

Select one:

a. polymorphisms

b. restriction enzymes

c. fluorescent primers

d. Southern blotting

14)
Name the process used to transfer the DNA fragments to a solid membrane.

Select one:

a. Southern blotting

b. PCR

c. gel electrophoresis

d. DNA restriction