11 Jun Irvine BIO 60080 – Describe the differences between
Question
Bio 1
Study Problems #3 (21pts)
1) Describe the differences between somatic cell nuclear tranfer, and induced pluripotent
stem cells (2pts)
2) What is a "pluripotent" cell? In an embryo, where do the pluripotent cells come from?
(2pts)
3) How does PCR work and why is it useful? (2pts)
4) What is recombinant DNA? How do restriction enzymes involved in this process? (2pts)
5) Restriction enzymes are extensively used in molecular biology. The recognition site of
EcoRI enzyme is 5’ G^AATTC 3’ .You are given a DNA sequence below. (3pts)
5’ATGAATTCGATCTACCGGAATTCGTAA3’
3’TACTTAAGCATCATGGCCTTAAGCATT5’
a. If this DNA sequence was cut with EcoRI, how many DNA fragments would you
expect?
b. Write out the sequence of these double-stranded DNA fragments.
6) If one parent is type A and the other is type B, but all four blood types are represented
among the children, what were the genotypes of the parents? (2pts)
7) Describe the three types of biodiversity. (3pts)
8) What does the Cas9 enzyme do to DNA when it binds to the DNA? Explain. (2pts)
9) A population has only two alleles, A and a, for a particular gene. The allele frequency of
a is 35%. How many would have the genotypes of AA, Aa, and aa in a population of
500? (3pts)
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