26 Jun Lab: Osmosis Osmosis, is a special form of diffusion
Question
Lab: Osmosis
Osmosis, is a special form of diffusion that occurs through a semi-permeable membrane. The membrane allows free
flow of the solvent (usually water), but blocks the passage of larger solute molecules, such as saccharose. The semipermeable
dialysis tubing that you will use simulates a cell membrane: only water and smaller molecules can diffuse
through the tubing.
Materials
dialysis tubing, three 250 mL beakers, syringe. scissors, water, thread, syrup, metric ruler, a balance Procedure
1. Label and prepare three beakers as follows.
Fill them about three-quarters full.
beaker #1: water
beaker #2: syrup
beaker #3: syrup
2. Cut off three segments (each about 10 cm long) of dialysis tubing. Submerse all the segments in a beaker with
water until you can see the tubing unfold. Fold one end of each segment back on itself (about 1 cm), and tie it tightly
with a string (see Fig. 2.1 below). Backfolding is important to prevent the “cells” from leaking.
3. Open the free end of one tube (call it tube #1) by moistening it and rubbing it between your fingers. Using the
syringe, fill it with the sugar solution (syrup). Squeeze out all of the air. Fold over the free end and tie it (like the
other end) with a thread. Rinse any syrup off the outside of the tube.
4. Fill tube #2 with sugar solution (syrup), and tie its free end as in step 3. Rinse as needed.
5. Fill tube #3 with water, and tie its free end as in step 3.
6. Place tube #1 in beaker #1, tube #2 in beaker #2, and tube #3 in beaker #3, as shown in Figure below. The liquids
in the beakers should cover the tubes completely. The sealed tubes form model cells.
7. Observe any changes in the model cells after one, two, and three hours, and note your observations in the
following table.
Model Cell #1 becomes hypotonic
Model Cell #2 Isotonic
Model Cell#3 becomes hypertonic
Treatment After one hour After two hours After three hours
Model Cell #1
Model Cell #2
Model Cell # 3
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