23 Jul LAC REPRESSOR BIND OPERATOR IN ABSENCE OF LACTOSE?
1. If the lac repressor protein is made in the presenc Show more For each of the following genotypes indicate: 1. If the lac repressor protein is made in the presence of lactose. 2. If the lac repressor protein is made in the absence of lactose. 3. If the lac repressor will bind to the lac operator in the presence of lactose 4. If the lac repressor will bind to the lac operator in the absence of lactose. 5. If -galactosidase will be produced in the presence of lactose 6. If -galactosidase will be produced in the absence of lactose 7. Circle any lac operon that can produce functional -galactosidase. Put an X through any lac operon that cannot produce a functional -galactosidase enzyme. (Circle or X out the entire lac operon; you should only have one mark for each DNA molecule) a. lacI+ lacP+ lacO+ lacZ+ 1. lac repressor protein made in presence of lactose? 2. lac repressor protein made in absence of lactose? 3. Lac repressor bind operator in presence of lactose? 4. Lac repressor bind operator in absence of lactose? 5. -galactosidase produced in presence of lactose? 6. -galactosidase produced in absence of lactose? 7. Circle lac operons that can produce -galactosidase put an X through any lac operons that cannot produce -galactosidase b. lac I+ lacP- lacOc lacZ+ 1. lac repressor protein made in presence of lactose? 2. lac repressor protein made in absence of lactose? 3. Lac repressor bind operator in presence of lactose? 4. Lac repressor bind operator in absence of lactose? 5. -galactosidase produced in presence of lactose? 6. -galactosidase produced in absence of lactose? 7. Circle functional lac operons put an X through non-function operons c. lacI- lacP+ lacOc lacZ- 1. lac repressor protein made in presence of lactose? 2. lac repressor protein made in absence of lactose? 3. Lac repressor bind operator in presence of lactose? 4. Lac repressor bind operator in absence of lactose? 5. -galactosidase produced in presence of lactose? 6. -galactosidase produced in absence of lactose? 7. Circle lac operons that can produce -galactosidase put an X through operons that cannot produce -galactosidase d. lacI+ lacP- lacO+ lacZ+ / lacI+ lacP+ lacOc lacZ- 1. lac repressor protein made in presence of lactose? 2. lac repressor protein made in absence of lactose? 3. Lac repressor bind operator in presence of lactose? 4. Lac repressor bind operator in absence of lactose? 5. -galactosidase produced in presence of lactose? 6. -galactosidase produced in absence of lactose? 7. Circle lac operons that can produce -galactosidase put an X through operons that cannot produce -galactosidase e. lacI- lacP+ lacO+ lacZ+ lacI+ lacP+ lacO+ lacZ- 1. lac repressor protein made in presence of lactose? 2. lac repressor protein made in absence of lactose? 3. Lac repressor bind operator in presence of lactose? 4. Lac repressor bind operator in absence of lactose? 5. -galactosidase produced in presence of lactose? 6. -galactosidase produced in absence of lactose? 7. Circle lac operons that can produce -galactosidase put an X through operons that cannot produce -galactosidase Show less
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