12 Jun Ligated Vibrio fischeri chromosomal DNA and pGEM plasmid
Question
(Ligated Vibrio fischeri chromosomal DNA and pGEM plasmid vector with Sal I, and transformed E.coli cells with recombinant plasmids. And then, checked if the cloning was successful. Blue colonies indicate that it contains only the plasmid, and white colonies indicate that the plasmid and inserts are sucessfully transformed into the E.coli, and if plasmid contain lux operon, it will glow.)
1. Why there are more blue colonies than white colonies on the plates? (%of white colonies were 0~11%)
2. Why so few of the white colonies glowing?
Our website has a team of professional writers who can help you write any of your homework. They will write your papers from scratch. We also have a team of editors just to make sure all papers are of HIGH QUALITY & PLAGIARISM FREE. To make an Order you only need to click Ask A Question and we will direct you to our Order Page at WriteDemy. Then fill Our Order Form with all your assignment instructions. Select your deadline and pay for your paper. You will get it few hours before your set deadline.
Fill in all the assignment paper details that are required in the order form with the standard information being the page count, deadline, academic level and type of paper. It is advisable to have this information at hand so that you can quickly fill in the necessary information needed in the form for the essay writer to be immediately assigned to your writing project. Make payment for the custom essay order to enable us to assign a suitable writer to your order. Payments are made through Paypal on a secured billing page. Finally, sit back and relax.
About Writedemy
We are a professional paper writing website. If you have searched a question and bumped into our website just know you are in the right place to get help in your coursework. We offer HIGH QUALITY & PLAGIARISM FREE Papers.
How It Works
To make an Order you only need to click on “Order Now” and we will direct you to our Order Page. Fill Our Order Form with all your assignment instructions. Select your deadline and pay for your paper. You will get it few hours before your set deadline.
Are there Discounts?
All new clients are eligible for 20% off in their first Order. Our payment method is safe and secure.