MIC 101 Exam 3

26 Jun MIC 101 Exam 3

Question
MIC 101 Exam 3 (01 December 2011)
• Please put your Name and Student ID Number on your ScanTron.
• Please fill-in Form A.
• Please mark your Answers to Multiple Choice Questions on your ScanTron.
• Please erase any Mistakes on your ScanTron very Carefully.

Please do not ask any Questions during the Exam.
___________________________________________________________________
1.

On a dark, Moonless Night you and your Clan Members jump the Fence of
the unspeakably disreputable Ming House of Swing and swipe a 50 ml Water
Sample from their Hot Tub. You use this Sample to perform a Most Probable
Number (MPN) Test:
Tube 1

Tube 2

Tube 3

10 ml (Double-Strength LSL Broth)

+

+

+

1.0 ml (Single Strength LSL Broth)

+

+

+

0.1 ml (Single Strength LSL Broth)

–

+

–

These MPN Test Results indicate that -A.

• The Most Probable Number (MPN) of Total Bacteria in this
Water Sample was 240 Bacteria per Milliliter of Water.
• The actual Number (“95% Confidence Limit”) was somewhere
between 35 and 470 Bacteria per Milliliter of Water.

B.

• The Most Probable Number (MPN) of Total Bacteria in this
Water Sample was 240 Bacteria per 100 ml of Water.
• The Number of Coliform Bacteria (“95% Confidence Limit”)
was somewhere between 36 and 1300 Coliform Bacteria per
100 Milliliters of Water.

C.

• The Most Probable Number (MPN) of Coliform Bacteria in this
Water Sample was 460 Coliform Bacteria per 100 ml of Water.
• The actual Number (“95% Confidence Limit”) was somewhere
between 71 to 2400 Coliform Bacteria per 100 ml of Water.

D.

• The Most Probable Number (MPN) of Total Bacteria in this
Water Sample was 460 Bacteria per Milliliter of Water.
• The Number of Coliform Bacteria (“95% Confidence Limit”)
was somewhere between 150 to 4800 Coliform Bacteria per
Milliliter of Water.

MIC 101 Exam 3 Page 1

2.

You leave the Lab to get a Drink of Water and when you return you discover
that your Lab Partner has added 9.0 ml of Sterile Saline to each of the 6 Test
Tubes of the Dilution Series for the Viable Count Experiment. Naturally, you
flunk your Oral Quiz and donʼt get the Ground Beast Sample. Everyone in Lab
points at you and laughs. Your Embarrassment is Profound and Complete.
Can you “Quick Fix” this by telling your TA youʼll be changing the Amounts
used to make and/or dispense the Ground Beast Dilutions?
A.

• A-OK! Affirmative!
• All youʼll need to do is transfer 200 µl of Ground Beast
Suspension from each Dilution Tube to each Nutrient Agar
Plate and MacConkey Agar Plate Pair (i.e. from the
10-7 Dilution Tube onto the two 10-8 Plates).
• Thatʼs it! With this one Change youʼre back in Business!
___________________________________________

B.

• You bet!
• All you need to do is transfer 1000 µl from the Initial 10-1
Ground Beast Sample to your 10-2 Dilution Tube and then
transfer 1000 µl at each Step in the Set-Up of the Dilution
Series (10-3, 10-4, 10-5, 10-6, 10-7).
• Thatʼs it! With this Change alone youʼre back in the Race!
___________________________________________

C.

• Can do!
• You will need to transfer 1000 µl from the Initial 10-1 Ground
Beast Sample to your 10-2 Dilution Tube and then transfer
1000 µl at each Step in the Set-Up of the Dilution Series
(10-3, 10-4, 10-5, 10-6, 10-7).
• You will also need to transfer 200 µl of Ground Beast Dilution
from each Dilution Tube to each Nutrient Agar Plate and
MacConkey Agar Plate Pair (i.e. from the 10-7 Dilution Tube
onto the two 10-8 Plates).
• It may be two (2) Changes but as long as you explain both
Changes to your Lab Partner using Words of no more than two
Syllables, youʼre still in Good Shape.
___________________________________________

D.

• Drat! No.
• Your only Option is to re-do the Dilution Series.

MIC 101 Exam 3 Page 2

3.

The Brothers Ho Kao and Lee Kao give you a 10 Gram Sample of the
Imitation Ground Beast they use in their Famous Ho-Lee Kao Quarter Pound
β-Burger. You blend this 10 Gram Sample in 90 ml of Sterile Saline and
perform a Viable Count. Your Results are:
Nutrient Agar Plates:
10-3

10-4

10-5

10-6

10-7

10-8

TNTC

TNTC

314

32

3

0

MacConkey Agar Plates:
10-3

10-4

10-5

10-6

10-7

10-8

TNTC

512

51

5

0

0

A Quarter Pound Burger should weigh 113 Grams. But Lee has been cutting
Costs by actually using an even 100 Grams of Imitation Ground Beast.
What is the approximate Total Number of Aerobic Bacteria that would be
present in an uncooked 100 Gram Ho-Lee Kao β-Burger made from this same
Batch of Imitation Ground Beast you tested?
A.
B.

3.2 x 1010 Bacteria

C.

5.1 x 108 Bacteria

D.
4.

3.2 x 109 Bacteria

5.1 x 109 Bacteria

After graduating from UC Davis you join the Peace Corps and are sent to
teach Microbiology in Kabul, Afghanistan. You decide to break the Ice with
your 24 Students by starting out with a Repeat of the Artificial Epidemic
(Jawa Fever) Exercise. Twenty-three (23) Students are given Tubes
containing Micrococcus. One (1) Student is given a Tube containing Serratia.
The Jawa Fever Exercise is performed exactly the same as when you took
MIC 101. Following incubation of all 24 Plates at 30°C for 48 Hours, how
many Plates should have a Red Patch in Quadrant I?
A.

1 Plate

B.

2 Plates

C.

4 Plates

D.

8 Plates

MIC 101 Exam 3 Page 3

5.

The Catalase Test -A.

B.

• Tests for the Presence of an Enzyme some Bacteria have in
their Electron Transport Chain.
• Catalase Positive Bacteria will fizz like Wombat Spit.

C.

• Tests for the Presence of an Enzyme some Bacteria have that
breaks-down Hydrogen Peroxide to Water and Oxygen.
• Catalase Positive Bacteria will turn Royal Thai Purple.

D.

6.

• Tests for the Presence of an Enzyme some Bacteria have that
breaks-down Hydrogen Peroxide to Water and Oxygen.
• Catalase Positive Bacteria will fizz like Wombat Spit.

• Tests for the Presence of an Enzyme some Bacteria have in
their Electron Transport Chain.
• Catalase Positive Bacteria will turn Royal Thai Purple.

The Oxidase Test -A.

• Tests for the Presence of an Enzyme some Bacteria have that
breaks-down Hydrogen Peroxide to Water and Oxygen.
• Oxidase Positive Bacteria will fizz like Wombat Spit.

B.

• Tests for the Presence of an Enzyme some Bacteria have in
their Electron Transport Chain.
• Oxidase Positive Bacteria will fizz like Wombat Spit.

C.

• Tests for the Presence of an Enzyme some Bacteria have that
breaks-down Hydrogen Peroxide to Water and Oxygen.
• Oxidase Positive Bacteria will turn Royal Thai Purple.

D.

• Tests for the Presence of an Enzyme some Bacteria have in
their Electron Transport Chain.
• Oxidase Positive Bacteria will turn Royal Thai Purple.

MIC 101 Exam 3 Page 4

7.

Pseudomonas was plated onto F Agar. This Agar is -A.
B.

Rich in Iron. As a Result Pseudomonas excretes Water-Soluble
Pigments in an Attempt to bind this Iron.

C.

Deficient in Iron. As a Result, Pseudomonas produces LipidSoluble Pigments for its Cell Membrane in an Attempt to bind
Iron.

D.

8.

Rich in Iron. As a Result, Pseudomonas produces Lipid-Soluble
Pigments for its Outer Membrane in an Attempt to bind this Iron.

Deficient in Iron. As a Result Pseudomonas excretes WaterSoluble Pigments in an Attempt to bind Iron.

In which of the Biochemical Tests was a Change of the pH Indicator to Yellow
considered a Positive Reaction?
A.
B.

Phenol Red-Mannitol

C.

Citrate

D.
9.

Indole

Vogues-Proskauer

In which of the Biochemical Tests were we assaying for the Ability to convert
Tryptophan in the Tryptone Broth to Ammonia and Pyruvic Acid?
A.
B.

Phenol Red-Mannitol

C.

Citrate

D.
10.

Indole

Vogues-Proskauer

In which of the Biochemical Tests was Bromthymol Blue the pH Indicator?
A.

Indole

B.

Phenol Red-Mannitol

C.

Citrate

D.

Vogues-Proskauer

MIC 101 Exam 3 Page 5

11.

Before any important Event the Ancient Romans would check the Flight,
Feeding and Entrails of Birds. Part of this was pure Roman Superstition (and
the Romans have always been very Superstitious), part was Roman Religion
(and the Romans have always been very Religious), part was merely to
satisfy the Romansʼ love of Grand Ceremonies (and Romans have always
been very Ceremonial). Which Characteristic Symptom of Avian Malaria could
have been observed by a properly trained Priest?
A.
B.

Hypoglycemia.

C.

Lymphadenopathy

D.

12.

Hepatomegaly.

Splenomegaly.

On 05 November 1880 the French Army Surgeon Alphonse Laveran
observed what he called Bodies Number 1, 2 and 3 inside Red Blood Cells on
Slides of Human Blood. Which Stages of Plasmodium could Laveran have
seen inside these Human Red Blood Cells?
A.
B.

Ookinetes and Oocysts.

C.

Trophozoites, Shizonts, and Merozoites.

D.

13.

Macrogametes and Sporozoites.

Trophozoites, Shizonts, and Sporozoites.

A Patient infected with Plasmodium falciparum experiences Fever on
Monday. This Fever will recur on -A.

Wednesday. The Fever is due to the Synchronous Release of
Merozoites from RBCs.

B.

Wednesday. The Fever is due to the Synchronous Release of
Sporozoites from Hepatocytes.

C.

Thursday. The Fever is due to the Synchronous Release of
Merozoites from RBCs.

D.

Thursday. The Fever is due to the Synchronous Release of
Sporozoites from Hepatocytes

MIC 101 Exam 3 Page 6

14.

Macrogametes and Microgametes of Plasmodium are produced when -A.

• Female Gametocytes and Male Gametocytes develop from
Merozoites inside separate Red Blood Cells in a Human.
• Female Gametocytes develop into non-motile Macrogametes
when ingested by a Mosquito.
• Male Gametocytes develop into 8 motile Microgametes when
ingested by a Mosquito.
• The Macrogamete is fertilized by a Microgamete.
• The Result is a Zygote.
__________________________________________________

B.

• Female Gametocytes and Male Gametocytes develop from
Merozoites inside the same Red Blood Cell in a Human.
• Female Gametocytes develop into non-motile Macrogametes
when ingested by a Mosquito.
• Male Gametocytes develop into 8 to 24 non-motile
Microgametes when ingested by a Mosquito.
• The Macrogamete is fertilized by a Microgamete.
• The Result is a Zygote.
__________________________________________________

C.

• Female Gametocytes and Male Gametocytes develop from
Merozoites inside separate Red Blood Cells only after they
have been ingested by a Mosquito.
• Female Gametocytes will then differentiate into a non-motile
Macrogamete.
• Male Gametocytes will then differentiate into 8 motile
Microgametes.
• The Macrogamete is fertilized by a Microgamete.
• The Result is a Zygote.
__________________________________________________

D.

• Female Gametocytes and Male Gametocytes develop from
Merozoites inside the same Red Blood Cell only after they
have been ingested by a Mosquito.
• Female Gametocytes will then differentiate into a motile
Macrogamete.
• Male Gametocytes will then differentiate into 8 to 24 non-motile
Microgametes.
• The Macrogamete is fertilized by a Microgamete.
• The Result is a Zygote.

MIC 101 Exam 3 Page 7

15.

What occurs after the Formation of an Oocyst?
A.
B.

Each non-motile Oocyst penetrates the Mosquito Midgut and
forms a motile Ookinete.

C.

• Each Oocyst undergoes Mitosis and forms ~ 10,000 motile
Ookinetes.
• These Ookinetes are released into the Hemocoel (Mosquito
Circulation) and migrate to and invade the Salivary Glands.

D.

16.

Each motile Oocyst penetrates the Mosquito Midgut and forms a
non-motile Ookinete.

• Each Oocyst undergoes Meiosis and forms ~10,000 motile
Sporozoites.
• These Sporozoites are released into the Hemocoel (Mosquito
Circulation) and migrate to and invade the Salivary Glands.

The Human Portion of the Plasmodium Life Cycle begins when a -A.
B.

Male Anopheles Mosquito “bites” and injects Merozoites,
which infect Red Blood Cells (RBCs) within 8 to 25 Days.

C.

Female Anopheles Mosquito “bites” and injects Sporozoites,
which infect Hepatocytes (Liver Cells) within about 30 Minutes.

D.

17.

Female Anopheles Mosquito “bites” and injects Merozoites,
which infect Hepatocytes (Liver Cells) within about 30 Minutes.

Male Anopheles Mosquito “bites” and injects Sporozoites,
which infect Red Blood Cells (RBCs) within 8 to 25 Days.

The Hypoglycemia that is a characteristic Symptom of Malaria is caused by -A.

The Loss of Hemoglobin to growing Merozoites.

B.

The Loss of Red Blood Cells upon Release of Merozoites.

C.

Rapidly growing Trophozoites.

D.

Developing Female Gametocytes and Male Gametocytes.

MIC 101 Exam 3 Page 8

Questions 18 – 23 cover Viral Hepatitis.

Viral Hepatitis
There are four important Viral Agents that can cause Hepatitis (G= inflammation of the
liver): Hepatitis A Virus, Hepatitis B Virus, Hepatitis C Virus and Hepatitis Delta
Virus. The Classic Symptoms of Hepatitis are extreme Fatigue, Anorexia, Nausea
(G= ship), Jaundice (French= yellow), and dark Urine. Excess Bile Pigments are
responsible for the Jaundice and dark Urine. The Manifestations of all Types of
Hepatitis are clinically Indistinguishable.
Hepatitis A
Hepatitis A Virus (HAV) [also known as Enterovirus 72] is a Picornavirus
(G= Small + RNA Virus) that causes Hepatitis A (sometimes known as Infectious
Hepatitis). Transmission is via the Fecal-Oral Route or via contaminated Shellfish.
Hepatitis A is sometimes called Short Incubation Hepatitis. The average Incubation
Time is a Month. Fewer than 50% of Hepatitis A Cases exhibit Classic Symptoms.
Diagnosis of Hepatitis A involves a dramatic Rise in Liver-associated Alanine
Aminotransferases (ALT). There are also Blood Tests specific for HAV Antigens or
HAV-Specific Antibodies (IgM). Treatment is Supportive. Most Cases resolve in 4 to
6 Weeks. There is an effective Inactivated Virus Vaccine (Havrix™).
Hepatitis A Virus infects approximately 1.5 Million People.
Hepatitis B
Hepatitis B Virus (HBV) is a Hepadnavirus (Hepatitis DNA Virus) that causes Hepatitis
B (sometimes known as Serum Hepatitis). Transmission is via contaminated Blood.
Hepatitis B Virus Levels in the Blood are extremely high. Since each Microliter of
Blood can contain up to a Million infectious HBV Particles, invisible Amounts of
Blood can transmit Hepatitis B. The Risk of Health Care Workers contracting HBV is
significantly greater than that of contracting HAV or HCV. The average Incubation
Period is two Months (but most Cases remain Subclinical). Hepatitis B is sometimes
called Long Incubation Hepatitis. Infection lasting longer than 6 Months is considered
Chronic Hepatitis. Approximately 10% of HBV Infections become Chronic.
Individuals with Chronic HBV Infection are 200 times more likely to develop
Primary Hepatocellular Carcinoma (PHC). The Distribution of HBV Infections and
PHC are nearly Identical. HBV is second only to Tobacco as a known Human
Carcinogen. Diagnosis involves a dramatic Rise in Liver-associated Alanine
Aminotransferase (ALT). There is a Blood Test for HBV Antigens or HBV-specific
Antibodies. Treatment is Supportive. There are two Recombinant Vaccines:
Engerix-B™ and Recombivax HB™. Hepatitis B Virus infects approximately
2 Billion (yes, that’s B Billion; approximately a Third of the World), mostly in
South Asia and Sub-Saharan Africa. Approximately 350 Million individuals are
Chronic HBV Carriers.

MIC 101 Exam 3 Page 9

Viral Hepatitis (continued)
Hepatitis C
Hepatitis C Virus (HCV) is a Flavivirus (L= yellow virus) that causes what was formerly
termed NANB Hepatitis (Non-A, Non-B Hepatitis). Transmission is most commonly
by contaminated Blood (as with Hepatitis B). Hepatitis C accounts for about 90% of
Hepatitis associated with Blood Transfusions. Transmission may also be by the FecalOral Route (like Infectious Hepatitis). The Incubation Period averages two Months
(but ~95% of Cases remain Subclinical and may remain Subclinical for at least as
long as 30 Years). Diagnosis involves dramatic Cyclic Fluctuations of Liverassociated Alanine Aminotransferases (ALT) and the Levels of these ALTs are
unusually High in HCV Infections. There are Blood Test for HCV Antigens and
HCV-Specific Antibodies. Treatment involves α-Interferon; however, the Side
Effects of α-Interferon Treatment are so severe that 10% of Patients cease taking it
(and α-Interferon Treatment only works in about 20% of Cases). There is no Vaccine
for Hepatitis C. Approximately 170 Million People are infected with HCV. Most
Infected Individuals are unaware that they have Hepatitis C; they usually find out
when they donate Blood and have it rejected
Hepatitis Delta Virus
Hepatitis Delta Virus (HDV) is a Satellite Virus (a Virus without enough Genetic
capacity to encode all its Components) that causes what was formerly termed Delta
Hepatitis. Hepatitis Delta Virus only infects Liver Cells infected with HBV. In effect,
HDV is slipstreaming HBV. Coinfection occurs if HBV and HDV are contracted
simultaneously; Superinfection occurs when HDV is contracted by an individual who
already has HBV (and that’s a pool of 350 Million). Coinfection generally resolves;
Superinfection frequently results in Chronic HDV Infection.

STOP!

• Do a Check of your ScanTron before you continue.
• You should have filled-in Answers through Question 17.
• The next Answer you fill-in should be for Question 18.

MIC 101 Exam 3 Page 10

18.

A single large Precursor Polyprotein is expressed from a large Open Reading
Frame (ORF) extending through most of the Hepatitis A Virus Genome. This
Polyprotein undergoes Nascent Cleavages (involving HAV Protease 2A and
HAV Protease 3C).
Post-Translational Cleavages (involving HAV Protease 3C) and a final
Maturation Cleavage result in a Total of -A.
B.

Three (3) Structural Proteins (VP1, VP2, VP0) and several
Non-Structural Proteins.

C.

Four (4) Structural Proteins (VP1, VP2, VP3, VP4) and
several Non-Structural Proteins.

D.

19.

Two (2) Structural Proteins (VPg and 3D) and several
Non-Structural Proteins.

Four (4) Structural Proteins (VPg, 2A, 3C, 3D) and several
Non-Structural Proteins.

Hepatitis B Virus (HBV) is a 42 nm Diameter Enveloped DNA Virus. The
Circular HBV Genome consists of a Negative Sense DNA Strand (3.2 Kb)
bound to a Positive Sense DNA Strand (1.7-2.8 Kb). The HVB Circular
Genome is said to be Asymmetrical because one Strand of the Genome is
significantly longer than the other Strand of the Genome. Since the Positive
Sense DNA Strand is shorter than the Negative Sense DNA Strand, between
15% and 50% of the Circular Genome is Single-Stranded DNA. The HBV
Genome is the smallest of all known Animal DNA Viruses. HBV is an
incredibly efficient Virus; every Base is used to code for at least one Protein
and many are involved in coding of more than one Protein.
The HBV DNA Polymerase is attached to the Positive Sense DNA Strand
inside the Virus Particle. This Polymerase was a huge Surprise, since it can
act as both a DNA-Dependent DNA Polymerase and an RNA-Dependent
DNA Polymerase.
Hepatitis B Virus is a -A.

Baltimore Class I Virus.

B.

Baltimore Class II Virus.

C.

Baltimore Class III Virus.

D.

Baltimore Class IV Virus.

MIC 101 Exam 3 Page 11

20.

Hepatitis B Virus does not grow in an any in vitro Cultured Cell Lines, so the
initial Steps of Virus Replication are poorly understood. A Number of
Candidate Receptors have been identified, including the Transferrin Receptor,
the Asialoglycoprotein Receptor Molecule and Human Liver Endonexin. It is
thought that HBV Surface Antigen (HBsAg) binds to a Receptor and interacts
with the Cell Membrane, exposing a Fusion Peptide. This results in the Fusion
of the HBV Envelope and the Host Cell Membrane and the Release of the
Nucleocapsid into the Cytoplasm. This Process is termed -A.
B.

Membrane Fusion.

C.

Receptor-Mediated Endocytosis.

D.

21.

Exocytosis.

Direct Entry.

Once the Nucleocapsid enters the Cytoplasm the Hepatitis B Virus DNA
Polymerase completes Positive Sense Strand Replication. The Asymmetrical
HBV Genome is converted into the Covalently Closed Circular DNA (cccDNA)
Form. This cccDNA Form is translocated to the Nucleus where it serves as
Template for HBV mRNA.
What Enzyme synthesizes HBV mRNA?
A.

Host Cell RNA Polymerase II uses the Positive Sense DNA
Strand as a Template to produce 3 HBV mRNAs that migrate to
the Cytoplasm, where HBV Proteins are synthesized.

B.

Host Cell RNA Polymerase II uses the Negative Sense DNA
Strand as a Template to produce 3 HBV mRNAs that migrate to
the Cytoplasm, where HBV Proteins are synthesized.

C.

HBV-Coded Reverse Transcriptase uses the Positive Sense
DNA Strand as a Template to produce 3 HBV mRNAs that
migrate to the Cytoplasm, where HBV Proteins are synthesized.

D.

HBV-Coded Reverse Transcriptase uses the Negative Sense
DNA Strand as a Template to produce 3 HBV mRNAs that
migrate to the Cytoplasm, where HBV Proteins are synthesized.

MIC 101 Exam 3 Page 12

22.

Assembly of Hepatitis C Virus Nucleocapsids occurs at the Cytoplasmic Face
of Rough Endoplasmic Reticulum (RER) Membranes, below Patches where
the glycoslylated HCV Envelope Proteins E1 and E2 have been inserted.
Multiple Copies of HCV Capsid Protein C and the Positive Sense RNA HCV
Genome assemble into Nucleocapsids. Encapsidation of HCV Genome
initiates a molding of the E1-E2-embedded Patches of RER Membrane
around the Nucleocapsid; HCV acquires its Envelope as it extrudes through
the RER Membrane into the Lumen of the RER.
Enveloped HCV Particles are transported via Vesicles from the RER to the
Cis-Face of the Golgi Membrane System — where E1 and E2 are
glycoslylated — after which they are transported via Vesicles from the
Trans-Face of the Golgi Membrane System to the Plasma Membrane, where
the enveloped HCV Particles are released.
The Process by which HCV is released from Hepatocytes is termed -A.
B.

Budding.

C.

Exocytosis.

D.
23.

Uncoating.

Membrane Fusion.

The Genome of Hepatitis Delta Virus (HDV) is an incredibly unique, Plant
Viroid-like, Single-Stranded Circular RNA Molecule with extensive internal
Base-Pairing to make it “look” just like Double-Stranded DNA to Host Cell
RNA Polymerase II (Pol II). Host Cell Pol II uses the HDV Genome as
Template for the Synthesis of the single HDV mRNA Molecule.
Host Cell Pol II also uses the HDV Genome as Template for making an
“Antigenome” that serves as a Template for making more HDV Genomes.
Hepatitis Delta Virus is a -A.
B.

Baltimore Class IV Virus.

C.

Baltimore Class V Virus.

D.
STOP!

Baltimore Class III Virus.

Baltimore Class VI Virus.

• Double-check your ScanTron for Errors.
• You have just answered the last of the Hepatitis Questions
(Question 23).
• Proceed to Polio (Question 24).

MIC 101 Exam 3 Page 13

24.

Polio did not become an Epidemic Disease until the Early Twentieth Century.
The First Polio Epidemics were in Scotland (1898), Sweden (1911) and the
US (1916). Paradoxically, these Polio Epidemics were associated with
improved Sanitation.
In Underdeveloped Areas Infants were raised under shockingly unsanitary
Conditions. These Infants were contracting Polio from ingesting other Infantsʼ
Feces. But we now know that the Majority of these Infants would have had -A.
B.

Abortive Polio or Non-Paralytic Polio.

C.

Non-Paralytic Polio or Aseptic Meningitis.

D.
25.

Asymptomatic or Abortive Infection.

Aseptic Meningitis or Paralytic Polio.

Headache, Fever, Sore Throat, Viremia and a protective IgG Response
would occur in approximately what Percentage of Polio Infections?
A.
B.

05%

C.

< 1%

D.
26.

95%

<< 0.1%

Does the Enzyme that synthesizes Polio Virus mRNA require a Primer?
A.

Yes. Poliovirus 3D requires VPg-UUUU (VPg-Poly-U) bound to
Poly-A at the 3´ End of the Replicative Intermediate to serve as
a Primer for mRNA Synthesis.

B.

No. Host Cell RNA Polymerase II does not require a Primer for
the Synthesis of these Positive Sense RNAs that can act as
(1) mRNA, (2) Template for more Replicative Intermediate
Synthesis or (3) Genome for Progeny Poliovirus.

C.

No. Poliovirus 3C, the RNA-Dependent RNA Polymerase
cleaved from Polyprotein P2, does not require a Primer for
mRNA Synthesis.

D.

No. Poliovirus 3D, the RNA-Dependent RNA Polymerase
cleaved from Polyprotein P3, does not require a Primer for
mRNA Synthesis.

MIC 101 Exam 3 Page 14

27.

The Poliovirus 247,000 Dalton Precursor Polypeptide undergoes Nascent
Cleavages by 2A and 3C to produce three (3) Polyproteins. Which of the
following best depicts the initial Post-Translational Cleavages of these
three (3) Polyproteins?
A.

• P1 VP1, VP3 and VP0
• P2 2A, VPg and 2C
• P3 3A, 3B, 3C and 3D
_______________________________

B.

• P1 VP1, VP3 and VP0
• P2 2A, 2B and 2C
• P3 3A, VPg, 3C and 3D
_______________________________

C.

• P1 VP1, VP2, VP3 and VP4
• P2 2A, VPg and 2C
• P3 3A, 3B, 3C and 3D
_______________________________

D.

28.

• P1 VP1, VP2, VP3 and VP4
• P2 2A, VPg and 2C
• P3 3A, 3B, 3C, 3CD and 3D

The Genome found inside a Polio Virus Particle was synthesized by -A.

• Poliovirus RNA Polymerase (3D) using the Positive Sense
Replicative Intermediate as a Template.
• VPg-AAAA (VPg-Poly-A) was the Primer.

B.

• Poliovirus RNA Polymerase (3D) using the