12 Jun Osmosis Lab Objective – Individual cells exist in a liquid
Question
Osmosis Lab
Objective: Individual cells exist in a liquid environment of about 75% water which makes it easier for food and other molecules to move into and out of a cell. Osmosis is the diffusion of water through a semi-permeable membrane. In osmosis, water moves to the area which is most concentrated. Cell membranes are selectively permeable, which means they allow some things to pass, but not everything. Water can pass across cell membranes, but not all molecules can. Water wants to move to an area that has a higher concentration of molecules. This lab serves to discover the effects of different concentrations of salt solutions on a plant root structure – in this case, a potato.
Materials needed:
• 1 fresh potato (old, mushy potatoes will not work!)
• Knife to cut potato
• 5 glasses or soup dishes big enough to hold a potato slice
• Water (from the tap)
• Salt
Procedure:
1. Fill each container with one cup of water.
2. Label the containers: 0 tsp; ¼ tsp; ½ tsp; 1 tsp; 2 tsp
3. Add salt in the amounts mentioned in item 2 to each container. e.g. container labeled ¼ tsp would have ¼ teaspoon of salt in the water, etc. Let stand and stir until salt is dissolved in each container.
4. Peel potato and cut into 5 – 1/8 “slices. If the slices don’t fit into your containers, trim them so they will. If you trim the slices, all of the slices have to be the same size to make your results valid.
5. Place a potato slice in each of the containers. Make a note what the slices look and feel like before you put them into the container. You can photograph these.
6. Formulate a hypothesis about what you think will happen to each of the potato slices.
7. Wait about 2 hours, and then check the slices and make notes about how they look and feel. Record the results. Describe your experiment. Compare your hypothesis (what you thought would happen) with what actually happened. Connect this to real world applications. What would happen if too much salt was accidentally spilled on one of your plants? What do you think would happen? Include photos of your experiment along with your description
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