11 Jun Penn BIO 110 -Serial Dilution Homework
Question
Serial Dilution Homework
Name:____________________________ Dilution = Section #:_________________________ 10-2 10-4 10-6 10-8 1. In the image above, the final dilution is 1:________________________. 2. You make six 2-fold serial dilutions of a solution. Your final dilution will be 1:__________. 3. You dilute 10 ?l of an unknown sample of bacteria to 1 x 10 -6 and plate out 10?l onto a Petri dish. The next
day you count 64 colonies. The number of viable bacteria in the original sample was
a. 6.4 x 106 cells/?l
b. 6.4 x 107 cells/?l
c. 6.4 x 108 cells/?l
d. 6.4 x 109 cells/?l
4. A 10 ?l sample of bacteria was diluted to 10-6 and 10?l
was plated on the Petri dish at right. What was the
approximate concentration of the original sample? 5. What are two reasons serial dilution is a useful technique for counting populations of bacteria? 1 2
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