26 Jun Protein synthesis worksheet
Question
BIO1
Name:
________________________________
Protein
Synthesis
Worksheet
You
isolate
the
following
piece
of
DNA
from
a
unicorn
hair
found
at
a
crime
scene.
3’TACAAATATAGACCTATAGAAAGCGGGATCCCATTCTACTGGCAATACCGCCTCTAC
AAAACTCTTTCGTTGGCGCGTTACCCACTCGCCCGCGGCCTCACTGGGTACCCACTCGC
CAGTTGCCGGGGGAGTTGCCCATA5’
1. Write
the
corresponding
second
strand
beneath
the
strand
above.
*Remember
to
include
the
polarity
of
the
second
strand!
2. The
promoter
and
termination
sequences
for
unicorns
are
as
follows:
Promoter
sequence:
AATATAGACCTATAGAA
Terminator
sequence:
CCGGGGGAGTTGCCC
Write
the
pre-‐mRNA
transcript
sequence
below.
*Remember
the
polarity!
3. What
are
the
three
post-‐transcriptional
modifications
eukaryotes
do
to
convert
a
pre-‐
mRNA
to
an
mRNA
transcript?
4. Unicorn
genes
use
the
following
introns:
Intron
1:
AUGGCGGAGAUGUUUUGA
Intron
2:
AUGGGUGAGCGG
Write
the
mRNA
transcript
below
remembering
to
remove
the
introns.
Spring
2014
1
BIO
1
5. Beginning
with
the
start
codon,
translate
the
mRNA
transcript
and
write
the
amino
acid
sequence
below.
Label
the
N
and
C-‐terminus
of
the
polypeptide.
6. Describe
the
process
by
which
a
ribosome
assembles
amino
acids
to
form
a
protein.
(Describe
the
three
steps
of
translation).
7. How
many
molecules
of
water
were
released
in
the
synthesis
of
the
above
protein?
8. Below
is
a
list
of
known
unicorn
genes
and
their
variants.
What
can
you
tell
about
the
missing
unicorn?
MET-‐THR-‐ASN-‐ASP-‐GLU-‐GLN-‐TRP-‐PHE-‐TYR-‐VAL-‐STOP
MET-‐THR-‐ASP-‐ASN-‐GLN-‐GLU-‐TRP-‐PHE-‐TYR-‐VAL-‐STOP
MET-‐THR-‐PRO-‐PHE-‐HIS-‐GLU-‐ALA-‐ARG-‐LEU-‐GN-‐STOP
MET-‐THR-‐PHE-‐PRO-‐HIS-‐GLN-‐ARG-‐ARG-‐ILE-‐GLY-‐STOP
MET-‐THR-‐SER-‐THR-‐ASP-‐VAL-‐ALA-‐ASP-‐VAL-‐ASN-‐STOP
MET-‐THR-‐SER-‐TYR-‐ASN-‐VAL-‐ALA-‐ASN-‐VAL-‐ASP-‐STOP
MET-‐THR-‐VAL-‐GLU-‐SER-‐ASN-‐ARG-‐ALA-‐ALA-‐PRO-‐GLU-‐STOP
MET-‐THR-‐VAL-‐GLU-‐SER-‐ASP-‐ASN-‐ALA-‐VAL-‐PRO-‐GLU-‐STOP
MET-‐CYS-‐PRO-‐LEU-‐LEU-‐LEU-‐THR-‐GLY-‐ASN-‐LYS-‐PRO-‐STOP
MET-‐CYS-‐PRO-‐ILE-‐LEU-‐LEU-‐TYR-‐GLU-‐ASN-‐LYS-‐PRO-‐STOP
Short
ears
Long
ears
Long
legs
Short
legs
Long
fur
Short
fur
Short
snout
Long
snout
Darker
fur
Lighter
fur
Spring
2013
2
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