28 Jun THE NUMBER OF CFU OF C. CAMELLENSUS
1. (21 points) The following table provides the data showing cell growth results obtained from a simple batch culture growth experiment. The bacterium Staphylococcus epidermidis was inoculated into 1 liter of culture medium and incubated at 37 °C for 24 hours. You are growing the S. epidermidis at its optimal temperature, 37°C in a nutrient medium at the optimal pH of 7.0. Cell numbers in the form of colony forming units (CFU/ml) were periodically determined. The results for the first 15 hours of the experiment are shown below in table 1.
Table 1: The number of CFU of C. camellensus
Time in Hours Colony Forming Units
0 22
1 24
2 23
3 72
4 280
5 1100
6 4350
7 17200
8 70900
9 285,000
10 1,100,500
11 4,500,000
12 4, 700,000
13 4, 520,000
14 3,800,000
15 1,700,000
16 820,000
1A) Plot the data given in the table using the semi log paper provided as a separate pdf file. Plot
time on the X axis and number of CFUs on the Y axis. Make sure to use a logarithmic scale on
the Y axis and label the axes. Be certain to label the X & Y axes and give your graph a title.
(8 points).
1B) Indicate on your graph which portions of the plot corresponds to the lag, exponential,
stationary, and death phases (2 points).
1C) Provide a description what is happening to the bacterial culture at these various phases in the growth curve giving the important attributes of each phase. (4 pts)
1D) Without using any equations, show on your graph how you can estimate the generation time
for this culture. You must briefly explain how you determined this. (3.5 points).
1E) Calculate the generation time for this culture using the equations discussed in class. In
other words, use the equation to determine the value of for the number of generations which
then can be used to determine the generation time. Show your work (3.5 points).
Reference: https://www.cabnr.unr.edu/howard/BCH406/How_to_use_Semi.doc
2) What changes would you see in the growth curve if you were to grow the organism in the same medium but with the pH changed to pH 6.0 and at a lower temperature of 30oC. In other
words, how would the growth curve appear different from the one that you plotted which was
done under optimal conditions? Explain. (3 pts)
Questions 3 (10 Points)
Discuss the advantages and disadvantages of active transport and group translocation. Please answer this in paragraph form, but if you use a Table to organize your thoughts include this as well.
Question 4 (4 points)
Related to question 3, compare the advantages and disadvantages of endocytosis to active transport and group translocation. Please answer this in paragraph form, but if you use a Table to organize your thoughts include this as well.
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