11 Jun The picture below is of a eukaryotic pre-mRNA
Question
6. The picture below is of a eukaryotic pre-mRNA that contains 4 exons (boxes) and 3 introns
(lines). An exonic splicing enhancer is present within exon 1 (starred box) – use of this enhancer
by a splicing activator is required in order for the 5’ splice site within intron 1 to be recognized
by the spliceosome. An intronic splicing silencer is located within intron 2 ( ). If this site is
used by a splicing repressor, the spliceosome can’t recognize the 3’ splice site of intron 2.
Remember that if one splice site isn’t used, the spliceosome doesn’t give up…it just looks for the
next splice site that COULD be used.
Exon 1 Exon 2 Exon 3 Exon 4 A) Cell type A contains a splicing repressor, but no splicing activator proteins. With respect to
the above pre-mRNA, draw a picture of the mature mRNA that will be present in cell type A? B) Cell type B contains both a splicing repressor protein and a splicing activator protein. With
respect to the above pre-mRNA, draw a picture of the mature mRNA that will be present in cell
type B?
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