26 Jun Using the DNA sequence
Question
WRITE ALL SEQUENCES 5’ TO 3’)
1. Using the DNA sequence Below : (5 pts)
5’-GTCAGCATGCGACTGTTGCTGCAAAAATGACCATG-3’
A. Write the complementary strand sequence:
B. Design a set of primers (6 nucleotides long) for synthesizing the top and bottom strands. To synthesize the Top:
To synthesize the Bottom:
C. If the original strand is the coding strand for a gene, and transcription begins at the first “G” nucleotide, what would the RNA sequence look like?
D. If the start of translation is the first AUG how many amino acids would this piece of DNA encode? E. What are the amino acids it would encode (write it N terminus to C terminus).
Use any amino acid codon chart you want.
2. Diagram the process by which eukaryotic mRNA is transcribed and processed to form the mature mRNA : (3 pt)
3. The model below shows the regulation of a gene in E. coli. It is shown under four different conditions and the resulting rates of transcription are reported. Each “condition” in the model is a different food source for the bacteria. Fill in the following sugars in their corresponding blanks in the model if the gene in question codes for the β-galactosidase enzyme. (The enzyme that cleaves Lactose into Glucose and Galactose) (7pts)
Condition A: Glucose and Lactose Condition C: Glycerol alone Condition B: Glucose alone Condition D: Lactose alone
Condition: A: _______________
B: _______________
C: _______________
D: _______________
sigma
Amount of transcript (compared to maximum)
5%
0.5%
0.5%
100%
A + B are “transcription regulator proteins”
RNA polymerase
A
B
E. Why should a sugar source influence the production of β-galactosidase?
F. Which transcription regulator is responding to the presence of glucose? _____ Explain your answer in one grammatically correct sentence.
G. Which transcription regulator is responding to the presence of lactose? _____ Explain your answer in one grammatically correct sentence.
Our website has a team of professional writers who can help you write any of your homework. They will write your papers from scratch. We also have a team of editors just to make sure all papers are of HIGH QUALITY & PLAGIARISM FREE. To make an Order you only need to click Ask A Question and we will direct you to our Order Page at WriteDemy. Then fill Our Order Form with all your assignment instructions. Select your deadline and pay for your paper. You will get it few hours before your set deadline.
Fill in all the assignment paper details that are required in the order form with the standard information being the page count, deadline, academic level and type of paper. It is advisable to have this information at hand so that you can quickly fill in the necessary information needed in the form for the essay writer to be immediately assigned to your writing project. Make payment for the custom essay order to enable us to assign a suitable writer to your order. Payments are made through Paypal on a secured billing page. Finally, sit back and relax.
About Writedemy
We are a professional paper writing website. If you have searched a question and bumped into our website just know you are in the right place to get help in your coursework. We offer HIGH QUALITY & PLAGIARISM FREE Papers.
How It Works
To make an Order you only need to click on “Order Now” and we will direct you to our Order Page. Fill Our Order Form with all your assignment instructions. Select your deadline and pay for your paper. You will get it few hours before your set deadline.
Are there Discounts?
All new clients are eligible for 20% off in their first Order. Our payment method is safe and secure.