23 Jul WHAT ARE THE ROLES OF THE OB AND DB GENES IN APPETITE REGULATION?
To study the roles of specific ge Show more What are the roles of the ob and db genes in appetite regulation? To study the roles of specific genes in regulating appetite researchers used laboratory mice with known mutations in those genes. Recessive mutations that inactivate both copies of either the ob gene or the db gene cause mice to eat voraciously and grow much more massive than wild-type mice. The researchers hypothesized that the wild-type ob and db genes participate in a hormone pathway that suppresses appetite when caloric intake is sufficient. To test this hypothesis they measured the mass of young mice (subject mice) of various genotypes and surgically linked the circulatory system of each subject mouse to that of another mouse. This procedure ensured that any substance circulating in the bloodstream of either mouse would be transferred to the other. All of the subject mice were given unlimited access to food and water. After eight weeks the researchers again measured the mass of each subject mouse. The table shows the data from the experiment. Part A Understanding the experimental design In this experiment mice of specific genotypes were paired together. Which of the following statements about the genotype pairings is correct? A. Pairing (a) joined two mice that were mutants for both the ob and db genes. B. Pairing (b) joined two mice that were mutants for the ob gene but wild-type for thedb gene. C. Pairing (c) joined a wild-type mouse with one that was a mutant for the db gene. D. Pairing (d) joined a wild-type mouse with one that was a mutant for the db gene. Part B In what way did pairing (a) serve as a unique experimental control? a. Only in pairing (a) were the subject mice joined surgically to other mice. b. Only in pairing (a) were the subject mice provided with unlimited access to food and water. c. Only in pairing (a) were both mice wild-type for both genes. d. Only in pairing (a) were changes in the body mass of the subject mice calculated. Part C Interpreting the data Based on the data shown in the table which of the following is a reasonable conclusion about the wild-type gene products? a. The ob + gene product circulated between both mice in a pair even when only one mouse was wild-type for that gene. b. The ob + gene product had no effect on the results of the experiment. c. The db + gene product had no effect on the results of the experiment. d. The ob + gene product did not circulate to the mutant mice when they were paired with wild-type mice. Show less
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