29 Jul WHAT DETERMINES A PERSONS ABILITY TO DIGEST LACTOSE?
What determines a persons ability to digest lactose?
B. Which of the wells showed a positive result for glucose? Explain the results.
Enzymes: Temperature, pH and Specificity Lab
7
C. Explain why testing for glucose is used to determine the activity of the enzyme
lactase.
D. Explain the experimental conditions for the five different wells.
Questions:
A. Graph the effect of temperature on the activity of the enzyme lactase.
B. What happens when an enzyme is boiled? Is this effect reversible?
C. Based on your experiment results, what is the optimal temperature for lactase
function?
D. Explain what happens as far as the effectiveness of the enzyme at the freezing
temperature.
Can this effect be overcome when the temperature rises?
Questions:
A. Graph the data placing glucose concentrations on the y-axis and the pH values on
the x-axis.
B. What was the effect of pH on the enzyme lactase? Is this true for all enzymes?
Questions:
Faith discussions promote an atmosphere of fellowship and
community (Rom 15:7). Please read the discussion topic
below and a post your comments. Comment and responses
are a minimum of a paragraph consisting of 3-5 lines.
Answer from mostly a human physiology standpoint but any
support from scriptures or faith would be additionally
beneficial.
Read Ps 31:10: My life is consumed by anguish and my years by groaning; my strength
fails because of my affliction and my bones grow weak. This verse describes how
despair and other negative feelings take a toll on our physical well-being. There are
many hypothalamic disorders that result in physical maladies such as wasting away
(cachexia), obesity, sleep disturbances, dehydration, as well as a wide range of
emotional disorders which over time causes the body great harm. Similarly, in Eccl 8:1:
Who is like the wise? Who knows the explanation of things? A persons wisdom
Enzymes: Temperature, pH and Specificity Lab
8
brightens their face and changes its hard appearance. Solomon describes how anxiety,
anger, sorrow and frustration can harden ones face, but enjoyment of life and attaining
wisdom will reverse these conditions.
1. Discuss how stress can causes excessive and prolonged cortisol release and the
disease state that may result from this.
2. What character in the bible suffered from severe sleep disturbances? Might this
be related to a hormonal imbalance? Previously, we discussed the viscera of the
abdominopelvic cavity. Splanchnon is the Greek word used to describe various
anatomical, physiological and emotional states. The viscera in the
abdominopelvic cavity have its own blood and nerve supply, aptly named the
splanchnic nerves and the splanchnic circulation. The splanchnic nerves are part
of the autonomic nervous system (ANS) and so the organs they innervate are not
directly under our voluntary control. However conscious emotional states
(anxiety, fear, happiness, etc.) can influence nervous output to the organs. In
addition, the adrenal medulla has the same embryological organ as the
sympathetic division of the ANS, however it releases epinephrine and
norepinephrine as hormones (instead of neurotransmitters) that circulate
throughout the blood stream contacting many organs. Interestingly, in a
theological context, splanchnon in the bible is related to emotions and is always
the responder. The Kardia refers to aspects of the mind and its control over
emotions. Thus, the Kardia is considered the originator.
3. Discuss the connection between mind and body, and how being in tune with
Gods will make us feel comfortable and confident as opposed to how we feel
when out of fellowship with the Lord.
CONCLUSION
SUMMARIZETHESEOBJECTIVESWITHBIGPICTURE
IDEA/12SENTENCESPEROBJECTIVE.
To learn how enzymes work
To understand enzyme specificity
To relate enzyme activity to temperature, pH, and concentration
To understand the role of enzymes in digestion of lactose
Enzymes: Temperature, pH and Specificity Lab
9
REFERENCES
Marieb & Hoehn (2010). Human Anatomy and Physiology.San Francisco, CA: Pearson
Benjamin Cummings
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