24 Jul WHAT IMPACT WILL THIS HAVE ON EXPRESSION OF LACZ?
If a mutation is introduced to the TrpL gene of the trp operon that results i Show more PICK ALL THAT APPLY. 1)If a mutation is introduced to the TrpL gene of the trp operon that results in a change of the tandem tryptophan codons to ones that code for tyrosine what impact will this have on expression of trpA? Compare this to a system with identical conditions except that there is no mutation. Check any and all that are true 0- TrpA expression levels will decrease 0- TrpA expression levels will not change 0- trpA expression regualtion will be regulated 0- TrpA expression levels will increase 0- trpA expression will be insensitive to tryptophan concentration 2) If a mutation is introduced to the CAP binding site of the lac operon that results in loss-of-function What impact will this have on expression of lacZ? Compare this to a system with identical conditions except that there is no mutation. Check any and all that are true 0- LacZ expression levels will decrease 0- lacZ expression will be constitutive 0- LacZ expression levels will increase 0- LazZ expression will be insensitive to lactose/allolactose concentration 0- LacZ expression will be regulated 0- LacZ expression levels will not change 0- LacZ expression will be insenstive to cAMP concentration 3) If a mutation is introduced to the lacY gene of the lac operon that results in loss-of-function what impact will this have on expression of lacZ? Compare this to a system with identical conditions except that theere is no mutation. Check any and all that are true 0- LazZ expression will be insensitive to lactose/allolactose concentration 0- lacZ expression will be constitutive 0- LacZ expression will be regulated 0- LacZ expression levels will decrease 0- LacZ expression levels will not change 0- LacZ expression will be insenstive to cAMP concentration 0- LacZ expression levels will increase 4) If a mutation is introduced to the promoter of the trp operon that results in a loss-of-funtion what impact will this have on expression of trpA? Compare this to a system with identical conditions except that there is no mutation. Check any and all that are true 0- trpA expression will be insensitive to tryptophan concentration 0- TrpA expression levels will increase 0- TrpA expression levels will not change 0- TrpA expression levels will decrease 0- trpA expression will be regulated 5) If a mutation is introduced to the lacI gene that results in loss-of-function what impact will this have on expression of lacZ? Compare this to a system with identical conditions except that there is no mutation. Check any and all that are true 0- LacZ expression will be regulated 0- LacZ expression levels will decrease 0- LacZ expression levels will not change 0- LacZ expression levels will increase 0- lacZ expression will be constitutive 0- LazZ expression will be insensitive to lactose/allolactose concentration 0- LacZ expression will be insenstive to cAMP concentration 6) If a mutation is introduced to the TrpR gene of the trp operon that results in a loss-of-funtion what impact will this have on expression of trpA? Compare this to a system with identical conditions except that there is no mutation. Check any and all that are true 0- TrpA expression levels will decrease 0- trpA expression will be insensitive to tryptophan concentration 0- TrpA expression levels will increase 0- trpA expression will be regulated 0- TrpA expression levels will not change Show less
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