23 Jul WHAT TYPES OF MOLECULAR MECHANISMS
Members Show more A virus MV-10 is known to be part of a larger family of dsDNA viruses the Mysterioviridae. Members of this family of viruses (not MV-10 itself) have been previously shown to exist in four different states: (a) episomally in the nucleus; (b) tethered to the telomeric region of a host chromosome; (c) fully-integrated as a provirus into the host genome; and (d) an active virion. MV-10 has broad tropism for different human cell types and after primary infection it appears to be latent for long periods of time. The triggers for reactivation are unknown. Furthermore it appears that in some cases the virus blebs from cells while in other cases host cell lysis has been observed. Your goal is to understand how the state of infection correlates to: productivity (i.e. virion yield); replication strategy (blebbing vs. lysis); and virulence. (A) Based on your general knowledge in virology and the main steps in virus life cycle what key assumptions would you make about the virus based on information given? Propose at least 4 characteristics that you would expect this virus to exhibit and why. (There is more than one set of correct answers. Choose carefully. These form the basis for your answers to the rest of the problem). i. ii. iii. iv. (B) It is unknown whether MV-10 establishes itself in one or more of states (a)-(c) above. (In other words the predominant state of the virus after primary infection is unknown). Design a set of experiments that would allow you to determine which state MV-10 establishes during primary infection and during latency. (C) How would you determine whether multiple states of infection can be present in a cell (i.e. an integrated or tethered copy as well as an episomal copy of the viral genome)? (D) Assume that from your experiments above that your data suggest that the virus can revert from a tethered (or integrated state) to an episomal state and that reactivation (i.e. active replication from latency) with high virion yields only occurs upon this conversion. What types of molecular mechanisms (e.g. enzymes) would you expect to find at play in this system and describe in step-by-step fashion how virus replication takes place upon reactivation. Show less
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