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WHATS THE HALF-LIFE (T½) FOR CASA SOLUTION AT 37°C AND PH 7?

WHATS THE HALF-LIFE (T½) FOR CASA SOLUTION AT 37°C AND PH 7?

Remove 0.25 mL sample from the flask after each hydrolysis time interval of approximately 45 min, and 90 min (record your exact time), dilute it to 10 mL with water, and analyze the diluted sample for SA concentration [M] at 310 nm; further dilution may be necessary to get absorbance values below 1.000.
4. Follow the hydrolysis reaction by analyzing all samples.
5. Record absorbance values and dilution factors (where required) for all samples.
6. Follow steps from 4 to 6 for the samples from the second temperature water bath.
Topics to be included in typed documents:
1. Results/Discussion
a. Determine the concentration [mM] of SA present in each hydrolysis sample at time X, (use the SA Calibration Curve)
b. Calculate the concentration [mM] of ASA remaining at each sample time by subtracting the SA conc [mM] (remember for each mole of ASA hydrolyzed, one mole of SA is formed) from the initial ASA concentration
c. Plot natural log (ln) ASA [mM] remaining vs. time (min) for each temperature
d. Determine the slope (= K app) of the line at each temperature
e. Determine Ea from the equation Log (K2/K1) = Ea(T2 – T1)/2.303xRxT2xT1
f. Plot natural log (ln) Kapp vs. 1/T (K) – temperature in Kelvin – and determine the slope = E a /R; determine the activation energy (E a ) for the hydrolysis of ASA; where R=1.987 cal/Kmole
g. Compare the Ea from the two methods
h. Determine the Knapp at body temperature (37°C) using the same equation as in e
i. Whats the half-life (T½) for CASA solution at 37°C and pH 7?
j. What is the relevance of this value?

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