10 Jun Which of the following genotypes would give the highest
Question
9. Which of the following genotypes would give the highest amount of lacZexpression, and why?
a) A mutation preventing transcription of lacI, the repressor, in glucose media
b) A mutation in the operator sequence in lactose media
c) A mutation in the CAP-recognition site in glucose
10. You are working on the chicken actin gene. You purify mRNA from chickens and run a Northern blot. You use a probe homologous to the 5′ end of the gene on the blot, and are surprised to see bands of three different sizes. Explain this data.
12. A mutation changes one amino acid in a protein sequence, located far away from the protein’s active site. Yet researchers working on this mutation discover that the mutated protein is nonfunctional. How?
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