Which reagent is added to S

•             Question 1

When performing the urease test, which of the following color changes is expected if the organism is positive for urease production?

 

Answers:

The agar changes from yellow to bright pink

The agar changes from red to yellow

The agar changes from blue to green

The agar changes from colorless to black

 

•             Question 2

 

 

The oxidase test determines the presence of cytochrome oxidase enzyme. This enzymes functions as part of _____________

 

Answers:

The electron transport chain

Glycolysis

The citric acid cycle

Fermentation

 

•             Question 3

 

 

Genotypic identification methods examine the DNA sequence of an organism

 

Answers:               True

False

 

•             Question 4

 

 

Which of the following would be most valuable to confirm the identification of microorganisms isolated during a food poisoning outbreak?

 

Answers:             Growth on agar plates to identify colony morphology

Grain stain to view cellular morphology

 

Identification of microorganisms using microscopic, cultural, and physiological characteristics

 

•             Question 5

 

 

Which of the following are examples of physiological (biochemical) characteristics used in the bacterial identification process?

 

Answers:             Gram stain

Cell shape

 

Presence of a specific enzyme

Growth in a nutrient broth

 

•             Question 6

 

 

Which methods used in bacterial identification usually require culturing of the unknown bacteria?

Answers:             Immunologic

Genotypic

 

Phenotypic

Morphologic

 

•             Question 7

 

 

If an organism produces stable acids as an end-product of glucose fermentation, the methyl red test will be red, indicating a positive result.

 

Answers:               True

False

 

•             Question 8

 

 

Which reagent is added to SIM medium to detect indole production?

Answers:             Methyl red reagent

Phenol red

Zinc powder

 

Kovac’s reagent

 

•             Question 9

 

 

In the Kligler’s Iron Agar (KIA) test, if an organism can only ferment glucose, the butt of the agar slant turns ______, and the slanted surface of the agar will be _______ after 24 hours of incubation.

Answers:             black; yellow

Red; yellow

 

Yellow; red

Yellow; black

 

•             Question 10

 

 

All of the following biochemical tests involve the growth of an organism in a specific liquid broth medium EXCEPT

Answers:             Nitrate reduction test

 

SIM test

Phenol red carbohydrate fermentation test

Methyl red test

 

•             Question 11

 

 

Which of the following is true of a bacterial species that produces dark colonies when grown on MacConkey agar.

 

Answers:             They are glucose fermenters

 

They are lactose fermenters

They produce urease

They reduce nitrate to nitrite

 

•             Question 12

 

 

The internationally recognized reference for bacterial classification and identification is called Bergey’s Manual

 

Answers:               True

False

 

•             Question 13

 

 

An organism has been shown to produce hydrogen sulfide and indole in its metabolism. These are examples of _____ characteristics that can be helpful in the identification of the microorganism.

Answers:             Morphological

Genotypic

 

Physiological

Immunological

 

•             Question 14

 

 

Which of the following types of media allows for the observations of multiple biochemical properties in one?

Answers:             Urea agar

MacConkey agar

 

SIM media

Citrate media

 

•             Question 15

Phenol red carbohydrate broths with Durham tubes can detect all of the following EXCEPT          Answers:

Nitrate reduction

Acid production

Gas production

Fermentation

 

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