23 Jul WHY DO THE TC CELLS LYSE THE TARGETS AND INDUCE DNA FRAGMENTATION?
Lymphocytes from a normal individual were stimulated in vitro by coculture with irradiated T lymphom Show more Lymphocytes from a normal individual were stimulated in vitro by coculture with irradiated T lymphoma cells. (Irradiation of these stimulator cells prevents them from dividing in the coculture.) After 7 days the lymphocytes were harvested and fractionated to obtain a population of cytotoxic Tcells (CD8+). These effector cells were set up in a cytotoxicity assay with the tumour cells as targets. The tumours cells were labeled in order to detect both DNA fragmentation and cell lysis. The following results were obtained in these assays: Treatment Lysis DNA Fragmentation No effector cells 4% 1% CD8+ Tc cells 82% 80% Anti-tumour cell antibody 5% 1% Anti-tumour cell antibody plus plasma 89% 1% Purified perforin 95% 2% Why do the Tc cells lyse the targets and induce DNA fragmentation? What are these cells recognizing on the tumour cell surface? Show less
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