25 Jun You perform a detailed kinetic of an enzyme in the presence and absence of an inhibitor.
Question
MCB100A_F2015_PS9.pages
19. You perform a detailed kinetic of an enzyme in the presence and absence of an inhibitor.
*Figure Attached* The data is shown below. Answer the following questions.
i. Using the plot above, solve for the Vmax and KM of the enzyme without inhibitor and the
apparent V*max and K*M upon addition of inhibitor.
ii. What is the mode of inhibition?
iii. What does this mechanism mean about drug binding with respect to the natural substrates?
1/v (sec/?M)
20. You are working with a homo-oligomeric enzyme and collect the following initial velocity data as a function of substrate concentration, [S].
[S] (mM)
v0
0.5
0.0667
1
0.1
2
0.2
5
0.5
10
1
a. What are the correct units for v0?
Carry out your kinetic analysis by constructing a Lineweaver-Burk plot. Note any special characteristics of your plot. What can you say about the fits of the Km and Vmax parameters?
Our website has a team of professional writers who can help you write any of your homework. They will write your papers from scratch. We also have a team of editors just to make sure all papers are of HIGH QUALITY & PLAGIARISM FREE. To make an Order you only need to click Ask A Question and we will direct you to our Order Page at WriteDemy. Then fill Our Order Form with all your assignment instructions. Select your deadline and pay for your paper. You will get it few hours before your set deadline.
Fill in all the assignment paper details that are required in the order form with the standard information being the page count, deadline, academic level and type of paper. It is advisable to have this information at hand so that you can quickly fill in the necessary information needed in the form for the essay writer to be immediately assigned to your writing project. Make payment for the custom essay order to enable us to assign a suitable writer to your order. Payments are made through Paypal on a secured billing page. Finally, sit back and relax.
About Writedemy
We are a professional paper writing website. If you have searched a question and bumped into our website just know you are in the right place to get help in your coursework. We offer HIGH QUALITY & PLAGIARISM FREE Papers.
How It Works
To make an Order you only need to click on “Order Now” and we will direct you to our Order Page. Fill Our Order Form with all your assignment instructions. Select your deadline and pay for your paper. You will get it few hours before your set deadline.
Are there Discounts?
All new clients are eligible for 20% off in their first Order. Our payment method is safe and secure.